Device
Part:BBa_K199095
Designed by: Mary Gearing Group: iGEM09_MoWestern_Davidson (2009-10-18)
pLac-RBS-CCACU-RFP-pTet-CCACU tRNA
Reporter construct ligated upstream of a suppressor tRNA. The tRNA suppresses the 5 bp insertion in the RFP gene.
This device works twice as well as its sister device of Part:BBa_K199094.
See Part:BBa_K199095:Experience link for characterization of this device.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1008
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 786
Illegal AgeI site found at 898
Illegal AgeI site found at 1045 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | pLac-RBS-CCACU-RFP-pTet-CCACU tRNA |